3QJP
An RAMP protein binding different RNA substrates
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.5621 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 76.781, 76.781, 193.078 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.295 - 3.299 |
| R-factor | 0.2312 |
| Rwork | 0.227 |
| R-free | 0.27170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.359 |
| Refinement software | PHENIX ((phenix.refine: dev_589)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.299 |
| Number of reflections | 9254 |
| Completeness [%] | 83.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 300 | 0.01 M MgSO4, 0.05 M Na Cacodylate (pH6.0), and 1.8 M Lithium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
