3QJL
One RAMP protein binding different RNA substrates
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.5621 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 88.773, 88.773, 257.629 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.700 - 2.701 |
R-factor | 0.2216 |
Rwork | 0.218 |
R-free | 0.26410 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.152 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: dev_589)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 28452 | |
Completeness [%] | 89.0 | 89 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 300 | 0.005 M MgSO4, 0.05 M MES (pH6.0), 3-8% PEG4000, 0.2 M NaCl and 0.1 M KCl, VAPOR DIFFUSION, HANGING DROP, temperature 300K |