3QJD
Human Hemoglobin A Mutant Alpha H58L Deoxy-Form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-06-21 |
Detector | RIGAKU RAXIS IV++ |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.650, 82.070, 53.490 |
Unit cell angles | 90.00, 99.51, 90.00 |
Refinement procedure
Resolution | 20.596 - 1.560 |
R-factor | 0.1696 |
Rwork | 0.169 |
R-free | 0.19070 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2dn2 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.193 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 61.790 | 61.790 | 1.500 |
High resolution limit [Å] | 1.450 | 3.120 | 1.450 |
Number of reflections | 76521 | ||
<I/σ(I)> | 13 | 1.9 | |
Completeness [%] | 80.9 | 98.7 | 14.2 |
Redundancy | 2.8 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.5 | 293 | 2.4-2.6 M ammonium phosphate/sulfate, pH 6.5, SMALL TUBES, temperature 293K |