3QIL
Crystal structure analysis of the clathrin trimerization domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Detector technology | CCD |
| Collection date | 2007-10-18 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.24 |
| Spacegroup name | H 3 |
| Unit cell lengths | 255.780, 255.780, 312.990 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.580 - 3.920 |
| R-factor | 0.339 |
| Rwork | 0.339 |
| R-free | 0.38600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.7LDz) |
| Phasing software | PHASER (1.3.2) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 500.000 | 54.580 | 3.880 |
| High resolution limit [Å] | 3.750 | 8.070 | 3.750 |
| Rmerge | 0.072 | 0.039 | 0.581 |
| Number of reflections | 61002 | ||
| <I/σ(I)> | 9.7 | 31.5 | 2.3 |
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 7.3 | 6.81 | 7.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | NaCl, Imidazole, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
