3QIJ
Primitive-monoclinic crystal structure of the FERM domain of protein 4.1R
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98322 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.001, 53.469, 88.766 |
Unit cell angles | 90.00, 106.96, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.209 |
Rwork | 0.207 |
R-free | 0.26150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gg3 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.464 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
Rmerge | 0.055 | 0.024 | 0.989 |
Number of reflections | 51901 | ||
<I/σ(I)> | 10.5 | ||
Completeness [%] | 99.7 | 98.6 | 97.1 |
Redundancy | 3.6 | 3.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 25% PEG3350, 0.2M magnesium chloride, 1% w/w dispase-I, pH 7.5, vapor diffusion, sitting drop, temperature 293K |