3QHZ

Crystal Structure of human anti-influenza Fab 2D1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	80
Detector technology	CCD
Collection date	2010-01-15
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	69.920, 80.940, 77.195
Unit cell angles	90.00, 100.14, 90.00

Refinement procedure

Resolution	28.085 - 1.550
R-factor	0.1809
Rwork	0.180
R-free	0.20620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3LZF (chains H and L)
RMSD bond length	0.006
RMSD bond angle	1.077
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.580
High resolution limit [Å]	1.550	1.550
Number of reflections	121518
Completeness [%]	98.7	96.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	22% PEG 4000 and 150 mM calcium acetate, VAPOR DIFFUSION, temperature 293K