3QHS
Crystal structure of full-length Hfq from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.910, 62.150, 81.260 |
| Unit cell angles | 78.56, 86.16, 59.86 |
Refinement procedure
| Resolution | 45.214 - 2.850 |
| R-factor | 0.20919 |
| Rwork | 0.207 |
| R-free | 0.24969 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hk9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.055 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 2.920 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.128 | 0.320 |
| Number of reflections | 23973 | |
| <I/σ(I)> | 14.22 | 5.6 |
| Completeness [%] | 92.9 | 83.7 |
| Redundancy | 7.03 | 6.43 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 30% v/v PEG 550 MME, 0.05M calcium chloride dihydrate, 0.1M BIS-TRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
