3QHS
Crystal structure of full-length Hfq from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.933 |
Spacegroup name | P 1 |
Unit cell lengths | 61.910, 62.150, 81.260 |
Unit cell angles | 78.56, 86.16, 59.86 |
Refinement procedure
Resolution | 45.214 - 2.850 |
R-factor | 0.20919 |
Rwork | 0.207 |
R-free | 0.24969 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hk9 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.055 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 2.920 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.128 | 0.320 |
Number of reflections | 23973 | |
<I/σ(I)> | 14.22 | 5.6 |
Completeness [%] | 92.9 | 83.7 |
Redundancy | 7.03 | 6.43 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 30% v/v PEG 550 MME, 0.05M calcium chloride dihydrate, 0.1M BIS-TRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |