3QHQ
Structure of CRISPR-associated protein Csn2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2010-11-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979981 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 75.300, 83.300, 110.400 |
| Unit cell angles | 90.00, 109.40, 90.00 |
Refinement procedure
| Resolution | 19.570 - 2.000 |
| R-factor | 0.2048 |
| Rwork | 0.204 |
| R-free | 0.22770 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.026 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.026 | 0.179 |
| Number of reflections | 43206 | |
| <I/σ(I)> | 36.51 | 7.39 |
| Completeness [%] | 99.4 | 97.9 |
| Redundancy | 4.53 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | 0.1M Hepes, 12% PEG 6000, 0.05M Phenole, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
