3QHP
Crystal structure of the catalytic domain of cholesterol-alpha-glucosyltransferase from Helicobacter pylori
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 35.786, 40.714, 53.738 |
Unit cell angles | 101.44, 94.87, 90.58 |
Refinement procedure
Resolution | 19.940 - 1.500 |
R-factor | 0.19757 |
Rwork | 0.196 |
R-free | 0.22734 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.315 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.141 | |
Number of reflections | 45557 | |
<I/σ(I)> | 8.6 | |
Completeness [%] | 96.0 | 94.3 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 297 | 0.1M HEPES (pH 7.5), 25% (w/v) PEG 3350, 0.1M glycine, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 297 | 0.1M HEPES (pH 7.5), 25% (w/v) PEG 3350, 0.1M glycine, VAPOR DIFFUSION, SITTING DROP, temperature 297K |