3QHF

Crystal Structure of Fab del2D1, a deletion variant of anti-influenza antibody 2D1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	80
Detector technology	CCD
Collection date	2010-03-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	68.913, 69.680, 97.678
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.655
R-factor	0.1845
Rwork	0.183
R-free	0.20920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3QHZ (chains H and L)
RMSD bond length	0.009
RMSD bond angle	1.227
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.710
High resolution limit [Å]	1.650	1.650
Number of reflections	55730
Completeness [%]	99.7	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		273	20% PEG 4000, 100 mM sodium acetate, and 200 mM magnesium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 273K