3QHE
Crystal structure of the complex between the armadillo repeat domain of adenomatous polyposis coli and the tyrosine-rich domain of Sam68
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-03-30 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 61 |
Unit cell lengths | 148.223, 148.223, 63.379 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 64.180 - 2.400 |
R-factor | 0.18915 |
Rwork | 0.187 |
R-free | 0.23046 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3au3 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.091 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.180 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.095 | 0.388 |
Number of reflections | 31344 | |
<I/σ(I)> | 7.1 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.2 | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 100mM BIS-TRIS (pH 5.5), 150mM NaCl, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |