3QGB

Crystal structure of FBF-2 R288Y mutant in complex with gld-1 FBEa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 61
Unit cell lengths	96.809, 96.809, 100.895
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.640 - 2.400
Rwork	0.168
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k5y
RMSD bond length	0.004
RMSD bond angle	0.607
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.640	2.440
High resolution limit [Å]	2.400	2.400
Rmerge		0.903
Number of reflections	21358
<I/σ(I)>	18.7	5.1
Completeness [%]	100.0	100
Redundancy	15.2	14.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	100 MM TRIS PH 7.5, 10% POLYETHYLENE GLYCOL 8000, AND 8% ETHYLENE GLYCOL, VAPOR DIFFUSION, HANGING DROP, temperature 298K