3QFQ

Asymmetric Assembly of Merkel Cell Polyomavirus Large T-antigen Origin Binding Domains at the Viral Origin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-10
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	P 21 21 2
Unit cell lengths	80.400, 171.340, 51.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.157 - 2.900
R-factor	0.2282
Rwork	0.225
R-free	0.28580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ntc
RMSD bond length	0.003
RMSD bond angle	0.710
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.000	3.060
High resolution limit [Å]	2.900	2.900
Rmerge	0.131	0.688
Number of reflections	15228
<I/σ(I)>	10.8	3.5
Completeness [%]	91.8
Redundancy	13	13.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	277	19-21% PEG 3350, 0.2M Lithium Acetate, 50 mM Tris pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 277K