3QFP
Crystal structure of yeast Hsp70 (Bip/Kar2) ATPase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2009-04-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9500 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.004, 64.053, 100.401 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.980 - 2.260 |
| R-factor | 0.23706 |
| Rwork | 0.235 |
| R-free | 0.26533 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | code 3LDN |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.890 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.294 | |
| Number of reflections | 17601 | |
| <I/σ(I)> | 19.2 | 3.71 |
| Completeness [%] | 86.8 | 51.9 |
| Redundancy | 4.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 298 | PEG 4000, pH 7, EVAPORATION, temperature 298K |
