3QEV

RB69 DNA Polymerase (L561A/S565G/Y567A) Ternary Complex with dCTP Opposite dT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	110
Detector technology	CCD
Collection date	2010-10-21
Detector	APEX II CCD
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	75.018, 119.553, 130.369
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.500 - 1.770
R-factor	0.17492
Rwork	0.173
R-free	0.20975
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.085
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.810
High resolution limit [Å]	1.770	1.770
Number of reflections	100984
Completeness [%]	94.8	82.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	150 mM CaCl2, 15% (w/v) PEG 350 monomethyl ether (MME), and 100 mM sodium cacodylate (pH 6.5), VAPOR DIFFUSION, temperature 298K