3QEF
The structure and function of an arabinan-specific alpha-1,2-arabinofuranosidase identified from screening the activities of bacterial GH43 glycoside hydrolases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-04 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 85.640, 85.640, 195.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.367 - 1.789 |
| R-factor | 0.1705 |
| Rwork | 0.169 |
| R-free | 0.19770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qed |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.142 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.370 | 1.890 |
| High resolution limit [Å] | 1.789 | 1.790 |
| Rmerge | 0.106 | 0.504 |
| Number of reflections | 69477 | |
| <I/σ(I)> | 16 | 5.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.5 | 14.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG3350, 200 mM ammonium tartrate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
