3QED

The structure and function of an arabinan-specific alpha-1,2-arabinofuranosidase identified from screening the activities of bacterial GH43 glycoside hydrolases

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-01
Detector	ADSC QUANTUM 315
Spacegroup name	P 41 21 2
Unit cell lengths	192.650, 192.650, 132.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.170 - 2.990
R-factor	0.20143
Rwork	0.200
R-free	0.23615
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.367
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELXS
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.170	3.150
High resolution limit [Å]	2.990	2.990
Rmerge	0.215	0.569
Number of reflections	50829
<I/σ(I)>	14.9	5.2
Completeness [%]	100.0	100
Redundancy	14.3	14.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1.5 M ammonium sulfate, 0.1 M Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K