3QE3
Sheep liver sorbitol dehydrogenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2010-01-01 |
Detector | RIGAKU SATURN 944+ |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 67.749, 85.881, 119.888 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.002 - 1.900 |
R-factor | 0.1945 |
Rwork | 0.192 |
R-free | 0.23430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pl8 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.077 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.064 | 0.508 |
Number of reflections | 27863 | |
<I/σ(I)> | 26 | 3.4 |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 2.3 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LASER-INDUCED NUCLEATION, SITTING DROP | 7 | 294 | 0.2 M lithium acetate dyhydrate, 20% PEG3350, pH 7.0, LASER-INDUCED NUCLEATION, SITTING DROP, temperature 294.0K |