3QDO

Crystal Structure of PDZ domain of sorting nexin 27 (SNX27) fused to the Gly-Gly linker followed by C-terminal (ESESKV) of GIRK3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-27
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	I 41
Unit cell lengths	59.795, 59.795, 53.606
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.280 - 1.880
R-factor	0.14688
Rwork	0.144
R-free	0.20305
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qe1
RMSD bond length	0.023
RMSD bond angle	2.020
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.280	1.910
High resolution limit [Å]	1.880	1.880
Rmerge	0.045	0.106
Number of reflections	7380
<I/σ(I)>	37.3	19.4
Completeness [%]	99.8	100
Redundancy	7.2	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.2 M calcium chloride, 0.1 M HEPES, 28% v/v PEG400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K