3QD9
C72S/C353S mutant of Trypanosoma brucei QSOX containing an interdomain disulfide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9769 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.040, 120.310, 106.220 |
| Unit cell angles | 90.00, 105.44, 90.00 |
Refinement procedure
| Resolution | 38.107 - 3.300 |
| R-factor | 0.196 |
| Rwork | 0.190 |
| R-free | 0.24860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qcp |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.828 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_629)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.095 | 0.355 |
| Number of reflections | 29261 | |
| <I/σ(I)> | 12.9 | 5 |
| Completeness [%] | 98.8 | 98.9 |
| Redundancy | 5.1 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% w/v PEG 3350, 0.2 M sodium sulfate, 0.5% w/v polyvinylpyrrolidone K15, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
