3QD7

Crystal structure of YdaL, a stand-alone small MutS-related protein from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-31
Detector	RAYONIX MX225HE
Wavelength(s)	0.9790
Spacegroup name	P 21 21 2
Unit cell lengths	95.517, 34.833, 40.698
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.760 - 2.300
R-factor	0.22516
Rwork	0.224
R-free	0.24358
Structure solution method	SAD, MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.367
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.061	0.169
Number of reflections	6306
<I/σ(I)>	24.3	8.26
Completeness [%]	100.0	98.2
Redundancy	5.9	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	289	1.5M ammonium sulfate, 0.1M Bis-Tris, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K
2	VAPOR DIFFUSION, HANGING DROP	6	289	1.5M ammonium sulfate, 0.1M Bis-Tris, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K