3QCR
Incomplete structural model of a human telomeric DNA quadruplex-acridine complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 71.674, 71.674, 29.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 9.960 - 3.200 |
| R-factor | 0.34595 |
| Rwork | 0.344 |
| R-free | 0.37837 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k8p |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.370 | 3.260 |
| High resolution limit [Å] | 3.160 | 3.160 |
| Rmerge | 0.102 | 0.115 |
| Number of reflections | 1104 | |
| <I/σ(I)> | 19.9 | |
| Completeness [%] | 97.5 | 100 |
| Redundancy | 16.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 285 | MPD, KCl, Na cacodylate, spermine, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
