3QCN
Human receptor protein tyrosine phosphatase gamma, domain 1, trigonal crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-27 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 75.200, 75.200, 152.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.490 - 2.410 |
| R-factor | 0.199 |
| Rwork | 0.198 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qcd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | HKL-2000 ((DENZO)) |
| Data scaling software | HKL-2000 ((SCALEPACK)) |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.9.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.500 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Rmerge | 0.186 | 0.537 |
| Number of reflections | 19097 | |
| <I/σ(I)> | 8 | 4.3 |
| Completeness [%] | 96.0 | 92 |
| Redundancy | 8.9 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7.0 |
