3QCB
Human receptor protein tyrosine phosphatase gamma, domain 1, apo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-15 |
Detector | RIGAKU SATURN 92 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.900, 79.500, 123.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.660 - 2.100 |
R-factor | 0.255 |
Rwork | 0.254 |
R-free | 0.30400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1RPM WITH LOOPS EXCISED AND NON- IDENTICAL SIDE CHAINS SHORN. |
RMSD bond length | 0.005 |
RMSD bond angle | 0.800 |
Data reduction software | HKL-2000 ((DENZO)) |
Data scaling software | HKL-2000 ((SCALEPACK)) |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.104 | 0.463 |
Number of reflections | 43827 | |
<I/σ(I)> | 18.8 | 3.2 |
Completeness [%] | 99.8 | 99 |
Redundancy | 6.4 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 277 | pH 6.5, vapor diffusion, temperature 277K |