3QC7
The structure of bacteriophage phi29 head fibers has a supercoiled triple repeating helix-turn-helix motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.968 |
Spacegroup name | H 3 2 |
Unit cell lengths | 47.248, 47.248, 424.752 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.470 - 1.520 |
R-factor | 0.19542 |
Rwork | 0.194 |
R-free | 0.21833 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.368 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.000 | 1.570 |
High resolution limit [Å] | 1.520 | 1.520 |
Rmerge | 0.070 | 0.407 |
Number of reflections | 28649 | |
<I/σ(I)> | 33.4 | 4.6 |
Completeness [%] | 98.6 | 97.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | The gp8.5 fragment crystals were grown in 100mM sodium phosphate at pH ~6.8 and 8% w/v PEG 3000. It took about one week for crystals to appear, VAPOR DIFFUSION, HANGING DROP, temperature 298K |