3QC1

Protein Phosphatase Subunit: Alpha4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-18
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97949
Spacegroup name	P 32 2 1
Unit cell lengths	76.600, 76.600, 72.680
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.362 - 2.350
R-factor	0.2148
Rwork	0.209
R-free	0.26700
RMSD bond length	0.007
RMSD bond angle	1.022
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.068	0.406
Number of reflections	12891
<I/σ(I)>	19.4	3.2
Completeness [%]	95.6	100
Redundancy	7	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	291	25% PEG 1500, pH 7.5, VAPOR DIFFUSION, temperature 291K