3QBZ
Crystal structure of the Rad53-recognition domain of Saccharomyces cerevisiae Dbf4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 83.740, 83.740, 103.669 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.823 - 2.692 |
R-factor | 0.2264 |
Rwork | 0.224 |
R-free | 0.27290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3oq4 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.034 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.740 |
High resolution limit [Å] | 2.690 | 2.690 |
Rmerge | 0.057 | 0.419 |
Number of reflections | 6371 | |
<I/σ(I)> | 31 | 3.5 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 6.9 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 1.9 M ammonium sulfate 50 mM sodium cacodylate 15 mM CYMAL-7, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |