3QB8

Paramecium Chlorella Bursaria Virus1 Putative ORF A654L is a Polyamine Acetyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Detector technology	CCD
Collection date	2010-08-12
Detector	MAR CCD 130 mm
Wavelength(s)	1.7
Spacegroup name	P 31
Unit cell lengths	65.400, 65.400, 112.631
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.278 - 1.500
R-factor	0.1526
Rwork	0.152
R-free	0.16800
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.693
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.400
Number of reflections	106487

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	295	0.9 M Sodium Citrate, 0.1M Imidizole pH 8.0, 0.1M Glycine-NaOH pH 10.5, 15% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 295K
1	VAPOR DIFFUSION, SITTING DROP	9.5	295	0.9 M Sodium Citrate, 0.1M Imidizole pH 8.0, 0.1M Glycine-NaOH pH 10.5, 15% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 295K