3QB5

Human C3PO complex in the presence of MnSO4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	77
Detector technology	CCD
Collection date	2010-06-21
Spacegroup name	P 61 2 2
Unit cell lengths	98.166, 98.166, 446.466
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.859 - 2.950
R-factor	0.226
Rwork	0.224
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pja
RMSD bond length	0.003
RMSD bond angle	0.610
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.940
Number of reflections	28514

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.4	293	1.2 M ammonium sulfate, 0.1 M phosphate citrate, 0.4 M lithium sulfate, soaked in 50 mM MnSO4, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K