3QB5
Human C3PO complex in the presence of MnSO4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2010-06-21 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 98.166, 98.166, 446.466 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.859 - 2.950 |
R-factor | 0.226 |
Rwork | 0.224 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3pja |
RMSD bond length | 0.003 |
RMSD bond angle | 0.610 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.940 |
Number of reflections | 28514 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 293 | 1.2 M ammonium sulfate, 0.1 M phosphate citrate, 0.4 M lithium sulfate, soaked in 50 mM MnSO4, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |