3QB0
Crystal structure of Actin-related protein Arp4 from S. cerevisiae complexed with ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-09 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 |
Unit cell lengths | 118.810, 118.810, 395.660 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.540 - 3.404 |
R-factor | 0.1929 |
Rwork | 0.192 |
R-free | 0.22070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yag |
RMSD bond length | 0.006 |
RMSD bond angle | 0.805 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.610 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.563 | |
Number of reflections | 43032 | |
<I/σ(I)> | 11.64 | 3.01 |
Completeness [%] | 99.5 | 98.4 |
Redundancy | 5.71 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 27 % PEG2000MME, 0.1 M HEPES-NaOH, 6 % D+-Trehalose, 50 mM Glycine, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |