3QAW
Crystal structure of a glutathione-S-transferase from Antarctic clam Laternula elliptica in a complex with glutathione
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2008-06-11 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 90.393, 57.611, 55.449 |
Unit cell angles | 90.00, 123.84, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.200 |
Rwork | 0.181 |
R-free | 0.21490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qav |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.860 |
Number of reflections | 11476 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 7% Tacsimate pH 8.0, 24% (w/v) polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K |