3QAN
Crystal structure of 1-pyrroline-5-carboxylate dehydrogenase from bacillus halodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-03 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.897, 75.559, 217.390 |
| Unit cell angles | 90.00, 98.48, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.950 |
| R-factor | 0.21817 |
| Rwork | 0.216 |
| R-free | 0.27963 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bhp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.255 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.870 | |
| Number of reflections | 120467 | |
| <I/σ(I)> | 5.2 | 1 |
| Completeness [%] | 99.6 | 97.1 |
| Redundancy | 4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 294 | 0.1M SODIUM ACETATE, PH 4.6, 200MM AMMONIUM SULFATE, 25% PEG4000, 10% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |
