3QAM
Crystal Structure of Glu208Ala mutant of catalytic subunit of cAMP-dependent protein kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-09-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.412, 79.639, 60.800 |
Unit cell angles | 90.00, 113.28, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.920 |
Rwork | 0.195 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1atp |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.990 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.051 | 0.440 |
Number of reflections | 32026 | |
<I/σ(I)> | 17 | 2.2 |
Completeness [%] | 88.8 | 65.5 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 10-12% polyethylene glycol 6000, 100 mM Tris-HCl, 10 mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |