3Q9Q
HspB1 fragment second crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-02-29 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 74.455, 74.455, 119.971 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.990 - 2.200 |
| R-factor | 0.2831 |
| Rwork | 0.281 |
| R-free | 0.31250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q9p |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.018 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.900 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 10572 | |
| <I/σ(I)> | 15.7 | 4 |
| Completeness [%] | 99.9 | 90 |
| Redundancy | 9.4 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 2.2M ammonium sulfate, 0.2M di-ammonium tartrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
