3Q9P
HspB1 fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-12-07 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9878 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 76.313, 76.313, 65.057 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.363 - 2.000 |
| R-factor | 0.2404 |
| Rwork | 0.237 |
| R-free | 0.27140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bol |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.834 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.400 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 10565 | |
| <I/σ(I)> | 23.9 | 4.7 |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 9.3 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 2.2M ammonium sulfate, 0.2M di-ammonium tartrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
