3Q9L
The structure of the dimeric E.coli MinD-ATP complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-04 |
| Detector | MAR 325 |
| Wavelength(s) | 0.9184,0.9788,0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.664, 86.595, 110.714 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.032 - 2.343 |
| R-factor | 0.2683 |
| Rwork | 0.266 |
| R-free | 0.30680 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.354 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.13) |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.032 | 100.000 | 2.430 |
| High resolution limit [Å] | 2.343 | 5.060 | 2.350 |
| Rmerge | 0.092 | 0.040 | 0.565 |
| Number of reflections | 33165 | ||
| <I/σ(I)> | 6.4 | ||
| Completeness [%] | 96.8 | 99.2 | 94.6 |
| Redundancy | 3.8 | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | 1.6 M ammonium sulfate, 0.1 M HEPES, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
