3Q98
Structure of ygeW encoded protein from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2009-11-13 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.97933 |
Spacegroup name | P 63 |
Unit cell lengths | 78.250, 78.250, 100.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.067 - 2.001 |
R-factor | 0.1791 |
Rwork | 0.175 |
R-free | 0.22550 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.056 |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.091 | 0.408 |
Number of reflections | 23411 | |
<I/σ(I)> | 15.5 | 5.8 |
Completeness [%] | 99.5 | 96.1 |
Redundancy | 9.1 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 20% PEG3350, 200 mM MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |