3Q91
Crystal Structure of Human Uridine Diphosphate Glucose Pyrophosphatase (NUDT14)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-02 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 84.040, 106.240, 106.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.170 - 2.700 |
| R-factor | 0.23054 |
| Rwork | 0.228 |
| R-free | 0.27137 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1G0S 1viq 1VIU and 2DSB |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.068 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.170 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.063 | 0.708 |
| Number of reflections | 26724 | |
| <I/σ(I)> | 8.5 | 2.1 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20 % w/v PEG3350, 0.2 M AMMONIUM FORMATE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
