3Q8T
Crystal structure of the coiled coil domain of Beclin 1, an essential autophagy protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-05 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.780, 38.350, 65.750 |
| Unit cell angles | 90.00, 97.92, 90.00 |
Refinement procedure
| Resolution | 35.920 - 1.900 |
| R-factor | 0.21024 |
| Rwork | 0.209 |
| R-free | 0.23631 |
| Structure solution method | MIR |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.243 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.920 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 19438 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 293 | 45% MPD, 2% PEG400, 100mM Tris, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
