3Q8I

Crystal structure of Anopheles Gambiae odorant binding protein 4 in complex with indole

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-11
Detector	"NOIR-1" MBC system
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	55.239, 66.364, 33.666
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.880 - 2.000
R-factor	0.20239
Rwork	0.200
R-free	0.24050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.465
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	14.880	2.070
High resolution limit [Å]	2.000	2.000
Rmerge		0.215
Number of reflections	8402
<I/σ(I)>		6.6
Completeness [%]	100.0	100
Redundancy	7.03	7.17

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.85	291	25.5% PEG, 100 mM MOPS, pH 6.85, VAPOR DIFFUSION, SITTING DROP, temperature 291K