3Q8I
Crystal structure of Anopheles Gambiae odorant binding protein 4 in complex with indole
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-11 |
Detector | "NOIR-1" MBC system |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 55.239, 66.364, 33.666 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.880 - 2.000 |
R-factor | 0.20239 |
Rwork | 0.200 |
R-free | 0.24050 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.465 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.880 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.215 | |
Number of reflections | 8402 | |
<I/σ(I)> | 6.6 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.03 | 7.17 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.85 | 291 | 25.5% PEG, 100 mM MOPS, pH 6.85, VAPOR DIFFUSION, SITTING DROP, temperature 291K |