3Q88
Glucose-6-phosphate isomerase from Francisella tularensis complexed with ribose 1,5-bisphosphate.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 114.465, 114.465, 84.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.990 - 1.700 |
R-factor | 0.1476 |
Rwork | 0.146 |
R-free | 0.17390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ljk |
RMSD bond length | 0.019 |
RMSD bond angle | 1.695 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.108 | 0.822 |
Number of reflections | 70571 | |
<I/σ(I)> | 10.1 | 2.69 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.2 | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | 0.2 M Ca(OAc)2, 0.1 M MES buffer, 10% 2-propanol, 0.01 M 5-phospho-alpha-D-ribose 1-diphosphate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |