3Q85
Crystal Structure of Rem2 G-domain -GTP Analog Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2008-11-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.525, 59.080, 56.144 |
Unit cell angles | 90.00, 101.26, 90.00 |
Refinement procedure
Resolution | 40.281 - 1.757 |
R-factor | 0.2092 |
Rwork | 0.208 |
R-free | 0.23530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cbq |
RMSD bond length | 0.005 |
RMSD bond angle | 1.019 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX ((AutoMR)) |
Refinement software | PHENIX ((phenix.refine: dev_412)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.790 |
High resolution limit [Å] | 1.757 | 4.780 | 1.757 |
Rmerge | 0.088 | 0.041 | 0.489 |
Number of reflections | 31389 | ||
<I/σ(I)> | 10.9 | 20.5 | 1.3 |
Completeness [%] | 93.2 | 97.2 | 58.1 |
Redundancy | 2.8 | 2.9 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 292 | 20%(w/v) PEG3350, 0.5M potassium thiocyanate and 7% glycerol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K |