3Q85

Crystal Structure of Rem2 G-domain -GTP Analog Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	110
Detector technology	CCD
Collection date	2008-11-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 1 21 1
Unit cell lengths	52.525, 59.080, 56.144
Unit cell angles	90.00, 101.26, 90.00

Refinement procedure

Resolution	40.281 - 1.757
R-factor	0.2092
Rwork	0.208
R-free	0.23530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cbq
RMSD bond length	0.005
RMSD bond angle	1.019
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX ((AutoMR))
Refinement software	PHENIX ((phenix.refine: dev_412))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.790
High resolution limit [Å]	1.757	4.780	1.757
Rmerge	0.088	0.041	0.489
Number of reflections	31389
<I/σ(I)>	10.9	20.5	1.3
Completeness [%]	93.2	97.2	58.1
Redundancy	2.8	2.9	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	292	20%(w/v) PEG3350, 0.5M potassium thiocyanate and 7% glycerol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K