3Q7Q

Crystal Structure of Rad G-domain Q148A-GTP Analog Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	110
Detector technology	CCD
Collection date	2008-11-21
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 31
Unit cell lengths	38.370, 38.370, 154.111
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.229 - 2.300
R-factor	0.1696
Rwork	0.166
R-free	0.20100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3q7p
RMSD bond length	0.004
RMSD bond angle	0.970
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX ((Auto.MR))
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.062	0.029	0.496
Number of reflections	11324
<I/σ(I)>	20.7	42.5	2.2
Completeness [%]	99.2	99.4	93.6
Redundancy	3.8	4.4	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.7	292	12% PEG 8000, 0.1M MES, 0.2M calcium acetate, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 292.0K