3Q7P
Crystal Structure of Rad G-domain-GTP Analog Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-11-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 31 |
| Unit cell lengths | 38.628, 38.628, 153.456 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.21422 |
| Rwork | 0.212 |
| R-free | 0.26270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB:2DPX |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.849 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.080 | 0.051 | 0.304 |
| Number of reflections | 8487 | ||
| <I/σ(I)> | 17.6 | 27.3 | 4.2 |
| Completeness [%] | 95.1 | 99.1 | 76.5 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 292 | 25% PEG 400, 5% ethylene glycol 0.1M MES, 0.2M calcium acetate,, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
