3Q7O
The crystal structure of BamB from the BAM complex in spacegroup P213
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 103.145, 103.145, 103.145 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.850 - 2.091 |
| R-factor | 0.2052 |
| Rwork | 0.199 |
| R-free | 0.26340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q7m |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.024 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_572)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.091 | 2.100 |
| Number of reflections | 21844 | |
| <I/σ(I)> | 12 | 1.8 |
| Completeness [%] | 98.9 | 98.9 |
| Redundancy | 7.5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 298 | 1.0 M lithium sulfate, 2% PEG8000, pH 7.5, temperature 298K |
