3Q7M

The crystal structure of BamB from the BAM complex in spacegroup I222

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-08-08
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97944, 0.97960, 0.94944
Spacegroup name	I 2 2 2
Unit cell lengths	50.318, 121.437, 134.291
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.378 - 1.651
R-factor	0.1645
Rwork	0.163
R-free	0.19250
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.065
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (- AutoSol - Solve)
Refinement software	PHENIX ((phenix.refine: dev_572))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.710
High resolution limit [Å]	1.650	1.650
Number of reflections	48256
<I/σ(I)>	24.1	1.6
Completeness [%]	99.0	99.9
Redundancy	5.1	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.18 M triammonium citrate, 20% PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K