3Q7M
The crystal structure of BamB from the BAM complex in spacegroup I222
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-08-08 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.97944, 0.97960, 0.94944 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 50.318, 121.437, 134.291 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.378 - 1.651 |
R-factor | 0.1645 |
Rwork | 0.163 |
R-free | 0.19250 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.065 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (- AutoSol - Solve) |
Refinement software | PHENIX ((phenix.refine: dev_572)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Number of reflections | 48256 | |
<I/σ(I)> | 24.1 | 1.6 |
Completeness [%] | 99.0 | 99.9 |
Redundancy | 5.1 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.18 M triammonium citrate, 20% PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |