3Q6V
Crystal Structure of Serratia fonticola Sfh-I: glycerol complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.830, 87.280, 72.570 |
| Unit cell angles | 90.00, 90.85, 90.00 |
Refinement procedure
| Resolution | 26.230 - 1.370 |
| R-factor | 0.1644 |
| Rwork | 0.163 |
| R-free | 0.19560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.322 |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.562 | 1.450 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.056 | 0.550 |
| Number of reflections | 84788 | |
| <I/σ(I)> | 3.3 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.1 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2M sodium acetate, 27% w/v PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
