3Q6T
Salivary protein from Lutzomyia longipalpis, Ligand free
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 120.385, 120.385, 248.117 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.930 |
| R-factor | 0.1965 |
| Rwork | 0.194 |
| R-free | 0.24586 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.630 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX (C) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 2.980 |
| High resolution limit [Å] | 2.930 | 7.870 | 2.930 |
| Rmerge | 0.095 | 0.030 | 0.647 |
| Number of reflections | 23723 | ||
| <I/σ(I)> | 13.3 | ||
| Completeness [%] | 99.9 | 98.5 | 100 |
| Redundancy | 14 | 12.2 | 14.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 298 | 10 % PEG 6000, 0.1M sodium citrate, 6 mM nickel chloride, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
