3Q6N
Crystal Structure of Human MC-HSP90 in P21 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-24 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 157.897, 90.868, 167.093 |
| Unit cell angles | 90.00, 115.85, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.050 |
| R-factor | 0.20721 |
| Rwork | 0.204 |
| R-free | 0.25921 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q6m |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.398 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.160 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.058 | 0.362 |
| Number of reflections | 82281 | |
| <I/σ(I)> | 19.6 | 5.1 |
| Completeness [%] | 98.8 | 99.4 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 1.0M ammonium sulfate, 0.04M Tris-sodium citrate, 0.04 mM cisplatin, pH 5.4-5.8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
