3Q6K
Salivary protein from Lutzomyia longipalpis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-29 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 120.012, 120.012, 244.747 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.520 |
| R-factor | 0.22039 |
| Rwork | 0.218 |
| R-free | 0.26787 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.405 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.560 |
| High resolution limit [Å] | 2.520 | 6.830 | 2.520 |
| Rmerge | 0.081 | 0.044 | 0.679 |
| Number of reflections | 35867 | ||
| <I/σ(I)> | 11.4 | ||
| Completeness [%] | 99.3 | 99.7 | 88.6 |
| Redundancy | 23 | 23.3 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 298 | 10 % PEG 6000, 0.1 M sodium citrate, 6 mM Nickel chloride, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
